CWC15 Polyclonal antibody

CWC15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25293-1-AP

Synonyms

Protein CWC15 homolog, ORF5, HSPC148, Cwf15, C11orf5

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Validation Data Gallery

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  WB analysis of mouse liver using 25293-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25293-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 25293-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25293-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25293-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25293-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25293-1-AP

  IP result of anti-CWC15 (IP:25293-1-AP, 3ug; Detection:25293-1-AP 1:600) with HeLa cells lysate 2800ug.

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  IHC staining of human stomach using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25293-1-AP (CWC15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25293-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25293-1-AP (CWC15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25293-1-AP targets CWC15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CWC15 fusion protein Ag18116
• Full Name: CWC15 spliceosome-associated protein homolog (S. cerevisiae)
• Calculated Molecular Weight: 229 aa, 27 kDa
• Observed Molecular Weight: 27-35 kDa
• GenBank Accession Number: BC040946
• Gene Symbol: CWC15
• Gene ID (NCBI): 51503
• RRID: AB_2880012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Publications

Species	Application	Title
	WB	Nucleic Acids Res; CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex.; Authors - Febe van Maldegem