CWC22 Polyclonal antibody

CWC22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26898-1-AP

Synonyms

Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL

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Validation Data Gallery

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  WB analysis of A549 using 26898-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26898-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26898-1-AP (CWC22 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26898-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 26898-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26898-1-AP (CWC22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26898-1-AP targets CWC22 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CWC22 fusion protein Ag25466
• Full Name: CWC22 spliceosome-associated protein homolog (S. cerevisiae)
• Calculated Molecular Weight: 908 aa, 106 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC093954
• Gene Symbol: CWC22
• Gene ID (NCBI): 57703
• RRID: AB_2880674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.

Publications

Species	Application	Title
human	WB	Science; Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.; Authors - Cole Townsend