CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2F5D5

Cat no : 60339-1-Ig

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

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Validation Data Gallery

• 

  WB analysis of PC-3 using 60339-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60339-1-Ig (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 60339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 60339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 60339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of PC-3 using 60339-1-Ig

  1X10^6 PC-3 cells were stained with 0.2 ug Anti-Human CX3CL1 (60339-1-Ig, Clone: 2F5D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human brain tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

60339-1-Ig targets CX3CL1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CX3CL1 fusion protein Ag0161
• Full Name: chemokine (C-X3-C motif) ligand 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001163
• Gene Symbol: CX3CL1
• Gene ID (NCBI): 6376
• RRID: AB_2881448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	JCI Insight; Single-cell transcriptome analysis and protein profiling reveal broad immune system activation in IgG4-related disease; Authors - Chenyang Lu

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