Anti-Human CX3CL1 Rabbit Recombinant Antibody

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• Anti-Human CX3CL1 Rabbit Recombinant Antibody (98595-2-RR)

CX3CL1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98595-2-RR

Clone No.251665D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

A-152E5.2, ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3C membrane-anchored chemokine

• 

  FC experiment of HepG2 using 98595-2-RR

  1x10^6 HepG2 cells were surface stained with 0.25 ug Anti-Human CX3CL1 Rabbit RecAb (98595-2-RR, Clone: 251665D12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98595-2-RR targets CX3CL1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6637

  Product name: recombinant human CX3CL1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 25-341 aa of BC016164

  Sequence: QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQ

• Full Name: chemokine (C-X3-C motif) ligand 1
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC016164
• Gene Symbol: CX3CL1
• Gene ID (NCBI): 6376
• RRID: AB_3746203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78423
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CX3CL1, also known as fractalkine, is a transmembrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).