CoraLite®594-conjugated CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F5D5

Cat no : CL594-60339

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

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Validation Data Gallery

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  FC experiment of PC-3 using CL594-60339

  1X10^6 PC-3 cells were stained with 0.4 ug CoraLite®594 Anti-Human CX3CL1 (CL594-60339, Clone:2F5D5) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: PC-3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60339 targets CX3CL1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CX3CL1 fusion protein Ag0161
• Full Name: chemokine (C-X3-C motif) ligand 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001163
• Gene Symbol: CX3CL1
• Gene ID (NCBI): 6376
• RRID: AB_2919919
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C