CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29819-1-AP

Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, RBS11, V28

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Validation Data Gallery

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  WB analysis using 29819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29819-1-AP (CX3CR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 29819-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (29819-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, THP-1 cells, Caco-2 cells
• Positive IF detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29819-1-AP targets CX3CR1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CX3CR1 fusion protein Ag27698
• Full Name: chemokine (C-X3-C motif) receptor 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa, 44 kDa
• GenBank Accession Number: BC028078
• Gene Symbol: CX3CR1
• Gene ID (NCBI): 1524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12). The two CX3CR1 bands exhibited can be attributed to various factors (PMID: 30628679).