CXADR Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86186-1-RR (same clone as 86186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86186-1-RR (same clone as 86186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 86186-1-RR (same clone as 86186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 86186-1-RR (same clone as 86186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86186-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86186-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86186-1-RR against Human CXADR were performed. The affinity constant is 96.1 pM.

CXADR Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86186-1-PBS

Clone No.250811C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

---

Product Information

86186-1-PBS targets CXADR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34346

  Product name: Recombinant human CXADR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC003684

  Sequence: MALLLCFVLLCGVVDFARSLSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQVIILYSGDKIYDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKIHLVVLVKPSGARCYVDGSEEIGSDFKIKCEPKEGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISVKNASSEYSGTYSCTVRNRVGSDQCLLRLNVVPPSNKAGLIAGAIIGTLLALALIGLIIFCCRKKRREEKYEKEVHHDIREDVPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQVPSEDFERTPQSPTLPPAKVAAPNLSRMGAIPVMIPAQSKDGSIV*

• Full Name: coxsackie virus and adenovirus receptor
• Calculated Molecular Weight: 365 aa, 40 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC003684
• Gene Symbol: CXADR
• Gene ID (NCBI): 1525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78310
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).