CXADR Recombinant antibody

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  WB analysis using 86186-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86186-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 86186-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 86186-1-RR

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86186-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86186-1-RR against Human CXADR were performed. The affinity constant is 96.1 pM.

CXADR Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 86186-1-RR

Clone No.250811C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

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Tested Applications

• Positive WB detected in: HaCaT cells, mouse heart tissue, rat heart tissue, mouse small intestine tissue, BxPC-3 cells
• Positive IHC detected in: mouse heart tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86186-1-RR targets CXADR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXADR fusion protein Ag34346
• Full Name: coxsackie virus and adenovirus receptor
• Calculated Molecular Weight: 365 aa, 40 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC003684
• Gene Symbol: CXADR
• Gene ID (NCBI): 1525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78310
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).