Anti-Mouse CXCL5 Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98417-2-RR

  1x10^6 HEK-293T cells (left) or CXCL5-transfected HEK-293T cells (right) with 0.25 ug Anti-Mouse CXCL5 Rabbit RecAb (98417-2-RR, Clone: 242737D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98417-2-RR

  1x10^6 HEK-293T cells (right) or CXCL5-transfected HEK-293T cells (left) with 0.25 ug Anti-Mouse CXCL5 Rabbit RecAb (98417-2-RR, Clone: 242737D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-2-RR, Clone: 240953A11) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CXCL5 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98417-2-RR

Clone No.242737D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

C-X-C motif chemokine 5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78), Scyb5

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98417-2-RR targets CXCL5 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXCL5 fusion protein Eg1636
• Full Name: chemokine (C-X-C motif) ligand 5
• Calculated Molecular Weight: 14KD
• GenBank Accession Number: NM_009141.3
• Gene Symbol: Cxcl5
• Gene ID (NCBI): 20311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50228
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.