- Featured Product
- KD/KO Validated
CXCR3B-specific Monoclonal antibody
CXCR3B-specific Monoclonal Antibody for FC, IHC, WB, ELISA
Cat no : 60065-1-Ig
Validation Data Gallery
|Positive WB detected in
|MOLT-4 cells, HT-29 cells, Jurkat cells, K-562 cells, U-937 cells
|Positive IHC detected in
|human tonsillitis tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60065-1-Ig targets CXCR3B-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2b
|chemokine (C-X-C motif) receptor 3
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.
Arterioscler Thromb Vasc Biol
Pericyte regulation of vascular remodeling through the CXC receptor 3.
Int J Obes (Lond)
Upregulation of angiostatic chemokines IP-10/CXCL10 and I-TAC/CXCL11 in human obesity and their implication for adipose tissue angiogenesis.
Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion.
Cell Commun Signal
Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer.
J Biol Chem
A novel CXCR3-B chemokine receptor-induced growth-inhibitory signal in cancer cells is mediated through the regulation of Bach-1 protein and Nrf2 protein nuclear translocation.
Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.