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  • KD/KO Validated

CXCR3B-specific Monoclonal antibody

CXCR3B-specific Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2D6

Cat no : 60065-1-Ig

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR



Tested Applications

Positive WB detected inMOLT-4 cells, HT-29 cells, Jurkat cells, K-562 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60065-1-Ig targets CXCR3B-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 46 kDa
Observed molecular weight43 kDa
GenBank accession numberAF469635
Gene symbol CXCR3
Gene ID (NCBI) 2833
RRIDAB_2292376
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

Protocols

Product Specific Protocols
WB protocol for CXCR3B-specific antibody 60065-1-IgDownload protocol
IHC protocol for CXCR3B-specific antibody 60065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arterioscler Thromb Vasc Biol

Pericyte regulation of vascular remodeling through the CXC receptor 3.

Authors - Bodnar Richard J RJ
  • KD Validated
humanWB,IF

Int J Obes (Lond)

Upregulation of angiostatic chemokines IP-10/CXCL10 and I-TAC/CXCL11 in human obesity and their implication for adipose tissue angiogenesis.

Authors - Luisa Hueso
humanWB, IF

Mol Cancer

Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion.

Authors - Wu Qian Q
humanFC

Cell Commun Signal

Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer.

Authors - Bo Ma
humanWB,FC

J Biol Chem

A novel CXCR3-B chemokine receptor-induced growth-inhibitory signal in cancer cells is mediated through the regulation of Bach-1 protein and Nrf2 protein nuclear translocation.

Authors - Murugabaskar Balan
  • KD Validated
mouseIF

Food Funct

Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.

Authors - Jinhong Xu