Biotin Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-98175

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7), 0.25 ug Biotin Anti-Human CXCR4/CD184 Rabbit RecAb (Biotin-98175, Clone: 241735F8) or unstained, and PE-conjugated streptavidin. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-98175

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CXCR4/CD184 Rabbit RecAb (Biotin-98175, Clone: 241735F8) (red) or unstained (blue), and PE-conjugated streptavidin. Cells were not fixed. Lymphocytes were gated.

CXCR4/CD184 Recombinant Antibody for FC

Cat No. Biotin-98175

Clone No.241735F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CXCR4, 241735F8, CD184, C-X-C chemokine receptor type 4, CXC R4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98175 targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated Molecular Weight: 352 aa, 40 kDa
• GenBank Accession Number: BC020968
• Gene Symbol: CXCR4
• Gene ID (NCBI): 7852
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61073
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).