CXXC5 Polyclonal antibody, PBS Only

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• CXXC5 Polyclonal antibody, PBS Only (16513-1-PBS)

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  WB analysis of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  WB analysis using 16513-1-AP (same clone as 16513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

  WB result of CXXC5 antibody (16513-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXXC5 transfected MCF-7 cells. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16513-1-PBS

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with SH-SY5Y cells lysate 1280 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 16513-1-AP (same clone as 16513-1-PBS)

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 16513-1-AP (same clone as 16513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16513-1-AP (same clone as 16513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 16513-1-AP (same clone as 16513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16513-1-AP (same clone as 16513-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CXXC5 antibody (16513-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 16513-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16513-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CF5, CXXC finger 5, CXXC-type zinc finger protein 5, HSPC195, Putative MAPK-activating protein PM08

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Product Information

16513-1-PBS targets CXXC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9733

  Product name: Recombinant human CXXC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-227 aa of BC017439

  Sequence: MMGGESADKATAAAAAASLLANGHDLAAAMAVDKSNPTSKHKSGAVASLLSKAERATELAAEGQLTLQQFAQSTEMLKRVVQEHLPLMSEAGAGLPDMEAVAGAEALNGQSDFPYLGAFPINPGLFIMTPAGVFLAESALHMAGLAEYPMQGELASAISSGKKKRKRCGMCAPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPSAALEKVMLPTGAAFRWFQ

• Full Name: CXXC finger 5
• Calculated Molecular Weight: 322aa,33 kDa; 227aa,24 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC017439
• Gene Symbol: CXXC5
• Gene ID (NCBI): 51523
• RRID: AB_2878269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7LFL8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.