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CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10894-1-AP

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10894-1-AP targets CYB5R3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag1339
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
WB protocol for CYB5R3 antibody 10894-1-APDownload protocol
IHC protocol for CYB5R3 antibody 10894-1-APDownload protocol
IF protocol for CYB5R3 antibody 10894-1-APDownload protocol
IP protocol for CYB5R3 antibody 10894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Calcium

Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.

Authors - D Marques-da-Silva
mouseIHC

JCI Insight

Loss of smooth muscle CYB5R3 amplifies angiotensin-II induced hypertension by increasing sGC heme oxidation.

Authors - Brittany G Durgin
mouseIHC

Blood Adv

Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.

Authors - Katherine C Wood
mouseWB

Geroscience

Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.

Authors - Sandra Rodríguez-López
mouseWB

Geroscience

CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.

Authors - Sara López-Bellón
mouseWB

Nitric Oxide

Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.

Authors - Elsayed M Mahgoup