CYB5R3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYB5R3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10894-1-AP

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

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Validation Data Gallery

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  WB analysis of HeLa using 10894-1-AP

  WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 10894-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10894-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10894-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10894-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10894-1-AP

  IP Result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 10894-1-AP

  Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

10894-1-AP targets CYB5R3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat, hamster, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag1339
• Full Name: cytochrome b5 reductase 3
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 30-35 kDa
• GenBank accession number: BC004821
• Gene symbol: CYB5R3
• Gene ID (NCBI): 1727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Publications

Species	Application	Title
rat	WB	Cell Calcium; Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.; Authors - D Marques-da-Silva
mouse	IHC	JCI Insight; Loss of smooth muscle CYB5R3 amplifies angiotensin-II induced hypertension by increasing sGC heme oxidation.; Authors - Brittany G Durgin
mouse	IHC	Blood Adv; Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.; Authors - Katherine C Wood
mouse	WB	Geroscience; Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.; Authors - Sandra Rodríguez-López
mouse	WB	Geroscience; CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.; Authors - Sara López-Bellón
mouse	WB	Nitric Oxide; Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.; Authors - Elsayed M Mahgoup