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CYB5R3 Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 10894-1-AP

Featured Product KD/KO validated

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Con: 27 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

CYB5R3 Antibody WB HeLa cells 10894-1-AP

WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells

CYB5R3 Antibody WB HepG2 cells 10894-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP(CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CYB5R3 Antibody IP HepG2 cells 10894-1-AP

IP Result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

CYB5R3 Antibody IHC human lung cancer tissue 10894-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP(CYB5R3 antibody) at dilution of 1:50 (under 10x lens)

CYB5R3 Antibody IF Hela cells 10894-1-AP

Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Species specificity:
human, mouse, pig, rat, hamster
Positive WB detected in:
HepG2 cells, human liver tissue, HeLa cells, A431 cells, MCF-7 cells, human placenta tissue, human lung tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CYB5R3 fusion protein Ag1339
Full name:
cytochrome b5 reductase 3
Calculated molecular weight:
33 kDa
Observed molecular weight:
30-35 kDa
GenBank accession number:
BC004821
Gene ID (NCBI):
1727
Gene symbol
CYB5R3
Synonyms
B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Background

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.


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