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- KD/KO Validated
CYB5R3 Polyclonal antibody
CYB5R3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10894-1-AP
|Positive WB detected in||HepG2 cells, human liver tissue, HeLa cells, A431 cells, MCF-7 cells, human placenta tissue, human lung tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10894-1-AP targets CYB5R3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|Tested Reactivity||human, mouse, pig, rat|
|Cited Reactivity||hamster, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CYB5R3 fusion protein Ag1339|
|Full Name||cytochrome b5 reductase 3|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||30-35 kDa|
|GenBank accession number||BC004821|
|Gene ID (NCBI)||1727|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.
|Product Specific Protocols|
|WB protocol for CYB5R3 antibody 10894-1-AP||Download protocol|
|IHC protocol for CYB5R3 antibody 10894-1-AP||Download protocol|
|IF protocol for CYB5R3 antibody 10894-1-AP||Download protocol|
|IP protocol for CYB5R3 antibody 10894-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.
Loss of smooth muscle CYB5R3 amplifies angiotensin-II induced hypertension by increasing sGC heme oxidation.
Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.
Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
J Comp Neurol
Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.
Mol Cell Neurosci
Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.