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  • KD/KO Validated

CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, pig, rat and More (1)





Cat no : 10894-1-AP


B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10894-1-AP targets CYB5R3 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag1339
Full Name cytochrome b5 reductase 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC004821
Gene Symbol CYB5R3
Gene ID (NCBI) 1727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.


Product Specific Protocols
WB protocol for CYB5R3 antibody 10894-1-APDownload protocol
IHC protocol for CYB5R3 antibody 10894-1-APDownload protocol
IF protocol for CYB5R3 antibody 10894-1-APDownload protocol
IP protocol for CYB5R3 antibody 10894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Transl Med

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes

Authors - Hitoshi Watanabe
  • KO Validated

J Cell Biol

Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.

Authors - Tim B Fessenden

JCI Insight

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling

Authors - Marta Bueno

Brain Struct Funct

High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.

Authors - A K Samhan-Arias

Blood Adv

Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.

Authors - Katherine C Wood

Free Radic Biol Med

Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3

Authors - Luz Marina Sánchez-Mendoza