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CYB5R3 Polyclonal antibody
CYB5R3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, human liver tissue, HeLa cells, A431 cells, MCF-7 cells, human placenta tissue, human lung tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 9 publications below |
IHC | See 4 publications below |
IF | See 4 publications below |
Product Information
10894-1-AP targets CYB5R3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | hamster, mouse, rat, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CYB5R3 fusion protein Ag1339 |
Full Name | cytochrome b5 reductase 3 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC004821 |
Gene symbol | CYB5R3 |
Gene ID (NCBI) | 1727 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.
Protocols
Product Specific Protocols | |
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WB protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IHC protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IF protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
IP protocol for CYB5R3 antibody 10894-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Calcium Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling. | ||
JCI Insight Loss of smooth muscle CYB5R3 amplifies angiotensin-II induced hypertension by increasing sGC heme oxidation. | ||
Blood Adv Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice. | ||
Geroscience Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice. | ||
J Comp Neurol Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain. | ||
Mol Cell Neurosci Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane. |