CYB5R3 Polyclonal antibody

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  WB analysis of HeLa using 10894-1-AP

  WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10894-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10894-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10894-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 10894-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10894-1-AP

  IP result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human normal colon using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10894-1-AP (CYB5R3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 10894-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (10894-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

CYB5R3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10894-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, human lung tissue, human liver tissue, mouse liver tissue, human placenta tissue, HeLa cells, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human normal colon, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 8 publications below

Product Information

10894-1-AP targets CYB5R3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag1339
• Full Name: cytochrome b5 reductase 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC004821
• Gene Symbol: CYB5R3
• Gene ID (NCBI): 1727
• RRID: AB_2292715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00387
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes; Authors - Hitoshi Watanabe ; KO Validated
		J Cell Biol; Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.; Authors - Tim B Fessenden
human,mouse	WB,IF	JCI Insight; CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling; Authors - Marta Bueno
rat	IHC	Brain Struct Funct; High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.; Authors - A K Samhan-Arias
mouse	IHC	Blood Adv; Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.; Authors - Katherine C Wood
mouse	WB	Free Radic Biol Med; Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3; Authors - Luz Marina Sánchez-Mendoza