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CoraLite®594-conjugated CYB5R3 Polyclonal antibody

CYB5R3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10894

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-10894 targets CYB5R3 in IF applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag1339
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL594 CYB5R3 antibody CL594-10894Download protocol
Standard Protocols
Click here to view our Standard Protocols