CYP11A1 Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 67264-1-Ig (same clone as 67264-1-PBS)

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67264-1-PBS in a different storage buffer formulation.

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  WB analysis using 67264-1-Ig (same clone as 67264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67264-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67264-1-Ig (same clone as 67264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67264-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67264-1-Ig (same clone as 67264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67264-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CYP11A1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67264-1-PBS

Clone No.1C5D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1C5D5, Cholesterol desmolase, Cholesterol side-chain cleavage enzyme, mitochondrial, CYP11A, CYPXIA1

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Product Information

67264-1-PBS targets CYP11A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4353

  Product name: Recombinant human CYP11A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC032329

  Sequence: MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRG

• Full Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Calculated Molecular Weight: 521 aa, 60 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC032329
• Gene Symbol: CYP11A1
• Gene ID (NCBI): 1583
• RRID: AB_2882535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05108
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.