CYP11A1 Monoclonal antibody

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• KD/KO Validated

CYP11A1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1C5D5

Cat no : 67264-1-Ig

Synonyms

Cytochrome P450 11A1, CYPXIA1, CYP11A, Cholesterol side-chain cleavage enzyme, mitochondrial, Cholesterol desmolase

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Validation Data Gallery

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  WB analysis of HepG2 using 67264-1-Ig

  WB result of CYP11A1 antibody (67264-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP11A1 transfected HepG2 cells.

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  WB analysis using 67264-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67264-1-Ig (CYP11A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67264-1-Ig (CYP11A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, SiHa cells, HeLa cells, Caco-2 cells. human placenta tissue, human testis tissue, rat testis tissue, mouse testis tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

67264-1-Ig targets CYP11A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP11A1 fusion protein Ag4353
• Full Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Calculated Molecular Weight: 521 aa, 60 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC032329
• Gene Symbol: CYP11A1
• Gene ID (NCBI): 1583
• RRID: AB_2882535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Reproduction; An ovarian phenotype of alpha 7 nicotinic receptor knockout mice; Authors - Pia Seßenhausen
mouse,rat	WB	Front Neurosci; Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats.; Authors - Tianying Pan
human	IHC	Reprod Sci; Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis; Authors - Haibo Zhang
		Cell Rep; Dissecting the spermatogonial stem cell niche using spatial transcriptomics; Authors - Shreya Rajachandran
mouse	IHC,WB	Toxicol Appl Pharmacol; Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice; Authors - Yong Zou
human	WB	Mol Hum Reprod; Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction; Authors - Maria Höfner