Tested Applications
| Positive WB detected in | LNCaP cells, HepG2 cells, SiHa cells, HeLa cells, Caco-2 cells. human placenta tissue, human testis tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human placenta tissue, rat adrenal gland tissue, mouse adrenal gland tissue, human testis tissue, mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67264-1-Ig targets CYP11A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4353 Product name: Recombinant human CYP11A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC032329 Sequence: MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRG Predict reactive species |
| Full Name | cytochrome P450, family 11, subfamily A, polypeptide 1 |
| Calculated Molecular Weight | 521 aa, 60 kDa |
| Observed Molecular Weight | 49-52 kDa |
| GenBank Accession Number | BC032329 |
| Gene Symbol | CYP11A1 |
| Gene ID (NCBI) | 1583 |
| RRID | AB_2882535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05108 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| WB protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP11A1 Monoclonal antibody (1C5D5), catalog number 67264-1-Ig, has 14 citations. These appear in 13 journals including Cell Reports, Environmental Pollution, Journal of Ethnopharmacology, Ecotoxicology and Environmental Safety, International Immunopharmacology, Molecular Human Reproduction, Frontiers in Neuroscience, Toxicology and Applied Pharmacology, Reproduction, Biology of Reproduction, Journal of Steroid Biochemistry and Molecular Biology, Reproductive Sciences, and Environmental Health. Research fields span steroidogenesis, reproductive endocrinology, testicular and ovarian biology, environmental toxicology, and neurodevelopment.
| Species | Application | Title |
|---|---|---|
Environ Pollut Arsenic exposure reduces testosterone synthesis partially by evoking Leydig cell ferroptosis in mouse testes. | ||
J Ethnopharmacol Saffron extract alleviates D-gal-induced late-onset hypogonadism by activating the PI3K-Akt-Nrf2 signaling pathway | ||
Ecotoxicol Environ Saf Gene-environment interaction in hypospadias: DEHP exposure aggravates DNAH8-related disruption of testicular steroidogenesis. | ||
Int Immunopharmacol Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary. | ||
Mol Hum Reprod Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction |
