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CYP19A1 Polyclonal antibody

CYP19A1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16554-1-AP

Synonyms

ARO, ARO1, Aromatase, CPV1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P 450AROM, Cytochrome P450 19A1, Estrogen synthase, P 450AROM



Tested Applications

Positive WB detected inA2780 cells, human testis tissue, mouse ovary tissue
Positive IHC detected inhuman gliomas tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skeletal muscle tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

16554-1-AP targets CYP19A1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity geese, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP19A1 fusion protein Ag9765
Full Name cytochrome P450, family 19, subfamily A, polypeptide 1
Calculated molecular weight 20 kDa, 25 kDa, 58 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC022896
Gene symbol CYP19A1
Gene ID (NCBI) 1588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharm Sci

PMID: 30776468

Authors - Vinay R Sonawane
geeseWB

Br Poult Sci

Effects of the FABP4 gene on steroid hormone secretion in goose ovarian granulosa cells.

Authors - Ruiming Fan
mouseWB, IF

Neuroscience

Colocalization of aromatase in spinal cord astrocytes: Differences in expression and relationship to mechanical and thermal hyperalgesia in murine models of a painful and a non-painful bone tumor.

Authors - E E O'Brien

Neuroscience

The relationship of bone-tumor-induced spinal cord astrocyte activation and aromatase expression to mechanical hyperalgesia and cold hypersensitivity in intact female and ovariectomized mice.

Authors - B A Smeester
mouseWB,IHC

J Ethnopharmacol

BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-κB pathways in aging mice induced by D-galactose and NaNO2.

Authors - Lin Li
mouseWB

Endocrinology

The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.

Authors - Qing He