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CYP19A1 Polyclonal antibody

CYP19A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16554-1-AP

Synonyms

ARO, ARO1, Aromatase, CPV1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P 450AROM, Cytochrome P450 19A1, Estrogen synthase, P 450AROM



Tested Applications

Positive WB detected inA2780 cells, mouse ovary tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, rat brain tissue, human kidney tissue, human skeletal muscle tissue, human gliomas tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16554-1-AP targets CYP19A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, geese
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP19A1 fusion protein Ag9765
Full Name cytochrome P450, family 19, subfamily A, polypeptide 1
Calculated molecular weight 20 kDa, 25 kDa, 58 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC022896
Gene symbol CYP19A1
Gene ID (NCBI) 1588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Protocols

Product Specific Protocols
WB protocol for CYP19A1 antibody 16554-1-APDownload protocol
IHC protocol for CYP19A1 antibody 16554-1-APDownload protocol
IF protocol for CYP19A1 antibody 16554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells.

Authors - Xue Jiao
mouseWB

Sci Rep

Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure.

Authors - Huidan Wang
mouse, ratWB

Sci Rep

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.

Authors - Jiansha Li
  • KD Validated
humanWB,IHC

Fertil Steril

Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.

Authors - Miao Sun
ratWB

Environ Pollut

Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation.

Authors - Zhiliang Li
mouseWB

Endocrinology

The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.

Authors - Qing He