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CYP19A1 Polyclonal antibody, PBS Only (16554-1-PBS)

Filter: AllWBIHCIF-PIF/ICC

WB analysis of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
WB analysis using 16554-1-AP (same clone as 16554-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16554-1-AP (same clone as 16554-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16554-1-AP (same clone as 16554-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16554-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CYP19A1 Polyclonal antibody, PBS Only

Name: CYP19A1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16554-1-PBS

CYP19A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 16554-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

ARO, ARO1, Aromatase, CPV1, CYAR

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16554-1-PBS targets CYP19A1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9765 Product name: Recombinant human CYP19A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC022896 Sequence: MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLLLFTPASVN Predict reactive species
Full Name	cytochrome P450, family 19, subfamily A, polypeptide 1
Calculated Molecular Weight	20 kDa, 25 kDa, 58 kDa
Observed Molecular Weight	55-58 kDa
GenBank Accession Number	BC022896
Gene Symbol	CYP19A1
Gene ID (NCBI)	1588
RRID	AB_2088670
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11511
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).