CYP1A2-Specific Recombinant monoclonal antibody, PBS Only

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  IHC staining of mouse liver using 84751-1-RR (same clone as 84751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84751-1-RR (same clone as 84751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84751-1-RR (CYP1A2-Specific antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84751-1-PBS in a different storage buffer formulation.

CYP1A2-Specific Uni-rAb^® Recombinant Antibody for IHC, Indirect ELISA

Cat No. 84751-1-PBS

Clone No.241922C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Indirect ELISA

Synonyms

CYP1A2, CYPIA2, CP1A2, CP12, Cholesterol 25-hydroxylase

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Product Information

84751-1-PBS targets CYP1A2-Specific in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 1, subfamily A, polypeptide 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_000761
• Gene Symbol: CYP1A2
• Gene ID (NCBI): 1544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.