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CYP1B1 Polyclonal antibody

CYP1B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 18505-1-AP


CP1B, CYP1B1, CYPIB1, Cytochrome P450 1B1, GLC3A, P4501B1

Tested Applications

Positive WB detected inPC-3 cells, MCF-7 cells, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18505-1-AP targets CYP1B1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1B1 fusion protein Ag13357
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012049
Gene Symbol CYP1B1
Gene ID (NCBI) 1545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).


Product Specific Protocols
WB protocol for CYP1B1 antibody 18505-1-APDownload protocol
IHC protocol for CYP1B1 antibody 18505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



NPJ Regen Med

Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration

Authors - Jingwen Hu


NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan

PLoS Pathog

Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.

Authors - Jillian A Bristol

Front Pharmacol

Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4+ T Cell Activities via Direct Binding With Cytochrome P450 1B1.

Authors - Shizhen Ding

J Transl Med

piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.

Authors - Qi Xie

Sci Rep

PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.

Authors - Jing Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hala (Verified Customer) (11-23-2021)

works very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepG2, hela cells