CYP1B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP1B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 18505-1-AP

Synonyms

Hydroperoxy icosatetraenoate dehydratase, GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1

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Validation Data Gallery

• 

  WB analysis using 18505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18505-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18505-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 18505-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18505-1-AP (CYP1B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 18505-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18505-1-AP (CYP1B1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells, mouse heart tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

18505-1-AP targets CYP1B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP1B1 fusion protein Ag13357
• Full Name: cytochrome P450, family 1, subfamily B, polypeptide 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012049
• Gene Symbol: CYP1B1
• Gene ID (NCBI): 1545
• RRID: AB_2878548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Publications

Species	Application	Title
mouse	IF	NPJ Regen Med; Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration; Authors - Jingwen Hu
human	WB	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
human	WB	PLoS Pathog; Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.; Authors - Jillian A Bristol
mouse	WB	Front Pharmacol; Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4+ T Cell Activities via Direct Binding With Cytochrome P450 1B1.; Authors - Shizhen Ding
human	WB	J Transl Med; piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.; Authors - Qi Xie
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (11-23-2021)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, hela cells