CYP1B1 Polyclonal antibody

CYP1B1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18505-1-AP

Synonyms

CP1B, CYP1B1, CYPIB1, Cytochrome P450 1B1, GLC3A, P4501B1



Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue, PC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18505-1-AP is CYP1B1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP1B1 fusion protein Ag13357
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Calculated molecular weight 61 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC012049
Gene symbol CYP1B1
Gene ID (NCBI) 1545
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Protocols

Product Specific Protocols
WB protocol for CYP1B1 antibody 18505-1-APDownload protocol
IHC protocol for CYP1B1 antibody 18505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharm Sci

PMID: 30776468

Authors - Vinay R Sonawane

Sensors (Basel)

Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink.

Authors - Damilare D Akintade
humanIF

Curr Med Chem

Ocular Ciliopathies: Genetic and Mechanistic Insights into Developing Therapies.

Authors - Mahesh Shivanna
humanWB

J Dermatol Sci

Role of the aryl hydrocarbon receptor signaling pathway in promoting mitochondrial biogenesis against oxidative damage in human melanocytes.

Authors - Xiaowen Wang