Tested Applications
| Positive WB detected in | SKOV-3 cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18505-1-AP targets CYP1B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13357 Product name: Recombinant human CYP1B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-259 aa of BC012049 Sequence: MGTSLSPNDPWPLNPLSIQQTTLLLLLSVLATVHVGQRLLRQRRRQLRSAPPGPFAWPLIGNAAAVGQAAHLSFARLARRYGDVFQIRLGSCPIVVLNGERAIHQALVQQGSAFADRPAFASFRVVSGGRSMAFGHYSEHWKVQRRAAHSMMRNFFTRQPRSRQVLEGHVLSEARELVALLVRGSADGAFLDPRPLTVVAVANVMSAVCFGCRYSHDDPEFRELLSHNEEFGRTVGAGSLVDVMPWLQYFPNPVRTVFR Predict reactive species |
| Full Name | cytochrome P450, family 1, subfamily B, polypeptide 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC012049 |
| Gene Symbol | CYP1B1 |
| Gene ID (NCBI) | 1545 |
| RRID | AB_2878548 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP1B1 antibody 18505-1-AP | Download protocol |
| WB protocol for CYP1B1 antibody 18505-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP1B1 Polyclonal antibody (Cat# 18505-1-AP) has 40 citations. These appear in journals including Cancer Cell, Metabolism, Redox Biology, Advanced Science, Cell Death & Differentiation, Phytomedicine, Journal of Translational Medicine, BMC Medicine, Fertility and Sterility, NPJ Regenerative Medicine, Journal of Ethnopharmacology, Biochemical Pharmacology, Frontiers in Endocrinology, International Journal of Molecular Sciences, International Immunopharmacology, Neurochemistry International, Frontiers in Pharmacology, mSystems, European Journal of Pharmaceutical Sciences, Molecular Medicine Reports, BBA Molecular Basis of Disease, Scientific Reports, PLoS Pathogens, Toxics, Cancers, Journal of Cellular and Molecular Medicine, Journal of Dermatological Science, iScience, Cancer Research Communications, Sensors, International Journal of Medical Sciences, Current Medicinal Chemistry, Tissue and Cell, Reproductive Sciences, Basic and Clinical Andrology, Journal of Gastrointestinal Oncology, Toxicological Sciences, and Pathology Research and Practice. Research fields span oncology, metabolism, immunology, neurology, toxicology, reproductive biology, and pharmacology.
| Species | Application | Title |
|---|---|---|
Cancer Cell Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8(+) T cell cytotoxicity and therapeutic response. | ||
Metabolism Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19 | ||
Redox Biol Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females | ||
Adv Sci (Weinh) Burn-Induced Gut Microbiota Dysbiosis Aggravates Skeletal Muscle Atrophy by Tryptophan-Kynurenine Mediated AHR Pathway Activation | ||
Cell Death Differ The AKR1C1-CYP1B1-cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma | ||
Phytomedicine Taxifolin promotes glioma stem cell differentiation via CYP1B1-mediated EMT suppression. |
Reviews
What customers say
The single available review rates the product 4 out of 5 stars. The user tested it by Western Blot on HepG2 and HeLa cells at a 1:1000 dilution and reported that it "works very good." Overall, the feedback is positive, indicating reliable performance for WB applications in these cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hala (Verified Customer) (11-23-2021) | works very good
|
