CYP20A1 Monoclonal antibody, PBS Only

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  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67433-1-PBS

Clone No.2A9E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2A9E2, CYP M, Cytochrome P450 20A1, EC:1.14.-.-, UNQ667/PRO1301

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Product Information

67433-1-PBS targets CYP20A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9715

  Product name: Recombinant human CYP20A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 112-462 aa of BC020616

  Sequence: SGGGSVSENHMREKLYENGVTDSLKSNFALLLKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGFLDGSLDKNMTRKKQYEDALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLASCIITAKLCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLRYCQHVLCETVRTAKLTPVSAQFQDIEGKIDRFIIPRETLVLYALGVVLQDPNTWPSPHKFDPDRFDDELVMKTFSSLGFSGTQECPELRFAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY

• Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC020616
• Gene Symbol: CYP20A1
• Gene ID (NCBI): 57404
• RRID: AB_2882671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q6UW02
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.