CYP20A1 Monoclonal antibody

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• CYP20A1 Monoclonal antibody (67433-1-Ig)

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67433-1-Ig Copied!

Clone No. 2A9E2

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA
For FC, select conjugated version.

Synonyms

2A9E2, CYP M, Cytochrome P450 20A1, EC:1.14.-.-, UNQ667/PRO1301

• 

  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 67433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 67433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 67433-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 67433-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, Neuro-2a cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67433-1-Ig targets CYP20A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9715

  Product name: Recombinant human CYP20A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 112-462 aa of BC020616

  Sequence: SGGGSVSENHMREKLYENGVTDSLKSNFALLLKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGFLDGSLDKNMTRKKQYEDALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLASCIITAKLCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLRYCQHVLCETVRTAKLTPVSAQFQDIEGKIDRFIIPRETLVLYALGVVLQDPNTWPSPHKFDPDRFDDELVMKTFSSLGFSGTQECPELRFAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY

• Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC020616
• Gene Symbol: CYP20A1
• Gene ID (NCBI): 57404
• RRID: AB_2882671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q6UW02
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody CYP20A1 (catalog no. 67433-1-Ig) has 1 total citation. It was used in Western Blot (WB) on human samples, published in Journal of Physiology and Biochemistry (IF 5.1, 2025). The associated research field is breast cancer, specifically investigating how microRNA-592 suppresses tumor progression by targeting CYP20A1 to modulate the CHOP pathway.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Physiol Biochem; MicroRNA-592 suppresses breast cancer progression by targeting CYP20A1 to modulate the CHOP pathway.; Authors - Ruolan Pan