CYP2S1 Polyclonal antibody

CYP2S1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19100-1-AP

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1

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Validation Data Gallery

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  WB analysis of COLO 320 using 19100-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19100-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19100-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 19100-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19100-1-AP (CYP2S1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse kidney tissue
• Positive IHC detected in: human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19100-1-AP targets CYP2S1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP2S1 fusion protein Ag4605
• Full Name: cytochrome P450, family 2, subfamily S, polypeptide 1
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033691
• Gene Symbol: CYP2S1
• Gene ID (NCBI): 29785
• RRID: AB_10640528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Pharmacol Rep; Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.; Authors - Hanna Szaefer