CYP4B1 Polyclonal antibody

CYP4B1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11771-1-AP

Synonyms

CYP4B1, CYPIVB1, Cytochrome P450 4B1, Cytochrome P450 HP, P 450HP

---

Validation Data Gallery

• 

  WB analysis using 11771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11771-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11771-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11771-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11771-1-AP (CYP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 11771-1-AP

  IP result of anti-CYP4B1 (IP:11771-1-AP, 4ug; Detection:11771-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 11771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11771-1-AP (CYP4B1 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse lung tissue, A549 cells, rat kidney tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11771-1-AP targets CYP4B1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP4B1 fusion protein Ag2416
• Full Name: cytochrome P450, family 4, subfamily B, polypeptide 1
• Calculated Molecular Weight: 512 aa, 59 kDa
• Observed Molecular Weight: 55-59 kDa
• GenBank Accession Number: BC017758
• Gene Symbol: CYP4B1
• Gene ID (NCBI): 1580
• RRID: AB_11182944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Hum Reprod; Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage; Authors - Shihua Bao