CYP4F11 Recombinant monoclonal antibody

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• CYP4F11 Recombinant monoclonal antibody (88260-1-RR)

CYP4F11 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88260-1-RR

Clone No.260726A10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

3-hydroxy fatty acids omega-hydroxylase CYP4F11, CYPIVF11, Cytochrome P450 4F11, Docosahexaenoic acid omega-hydroxylase, EC:1.14.14.1

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  WB analysis using 88260-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88260-1-RR (CYP4F11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88260-1-RR targets CYP4F11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2906

  Product name: Recombinant human CYP4F11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 175-524 aa of BC016853

  Sequence: HDKWQRLASEGSARLDMFEHISLMTLDSLQKCVFSFESNCQEKPSEYIAAILELSAFVEKRNQQILLHTDFLYYLTPDGQRFRRACHLVHDFTDAVIQERRRTLPTQGIDDFLKNKAKSKTLDFIDVLLLSKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQEQCRQEVQELLKDREPIEIEWDDLAQLPFLTMCIKESLRLHPPVPVISRCCTQDFVLPDGRVIPKGIVCLINIIGIHYNPTVWPDPEVYDPFRFNQENIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALTLLHFRILPTHTEPRRKPELILRAEGGLWLRVEPLGANSQ

• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 11
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC016853
• Gene Symbol: CYP4F11
• Gene ID (NCBI): 57834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HBI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)