CYP4F11-Specific Polyclonal antibody

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  IHC staining of human hepatocirrhosis using 20012-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20012-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20012-1-AP (CYP4F11-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20012-1-AP

  Immunofluorescent analysis of HepG2 cells, using CYP4F11 antibody 20012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CYP4F11-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 20012-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

CYP4F11, CYP4F11-Specific, CYPIVF11, Cytochrome P450 4F11

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Tested Applications

• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20012-1-AP targets CYP4F11-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 11
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001128932
• Gene Symbol: CYP4F11
• Gene ID (NCBI): 57834
• RRID: AB_10665946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F11 belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F11 catalyze the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP4F11. It has no cross reaction to other CYP4F member.This antibody is specific to CYP4F11.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse		Int Immunopharmacol; Regulation of cytochrome P450 4F11 expression by liver X receptor alpha.; Authors - Tao Zhang
human,mouse	IHC	Biochem Biophys Rep; Targeting estrogen mediated CYP4F2/CYP4F11-20-HETE metabolic disorder decelerates tumorigenesis in ER+ breast cancer; Authors - Juan Yang