CYP4F2-Specific Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Hepatocellular Cancer using 85320-1-RR (same clone as 85320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

CYP4F2-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85320-1-PBS

Clone No.242307B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase

---

Product Information

85320-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: NM_001082
• Gene Symbol: CYP4F2
• Gene ID (NCBI): 8529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78329
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).