CYP4F2-Specific Recombinant antibody

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  WB analysis using 85320-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85320-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CYP4F2-Specific Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85320-1-RR

Clone No.242307B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CYP4F2, CPF2, Arachidonic acid omega-hydroxylase, 242307B10, 20-hydroxyeicosatetraenoic acid synthase

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, A549 cells, HuH-7 cells, SMMC-7721 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85320-1-RR targets CYP4F2-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: NM_001082
• Gene Symbol: CYP4F2
• Gene ID (NCBI): 8529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78329
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).