CYP4F3 Polyclonal antibody

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• CYP4F3 Polyclonal antibody (19701-1-AP)

CYP4F3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19701-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CPF3, CYP4F, CYPIVF3

• 

  WB analysis using 19701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19701-1-AP (CYP4F3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovarian cancer using 19701-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19701-1-AP (CYP4F3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, HepG2 cells
• Positive IHC detected in: human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19701-1-AP targets CYP4F3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 3
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000896
• Gene Symbol: CYP4F3
• Gene ID (NCBI): 4051
• RRID: AB_2878599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08477
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F3, also named as LTB4H and CYPIVF3, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F3 requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.

Publications

What published studies show

The CYP4F3 Polyclonal antibody (Cat# 19701-1-AP) has 2 total citations. One appeared in J Cancer Res Clin Oncol, studying probiotic therapy's protective effect on gut microbiota and ω-oxidation activation after ovarian cancer surgery in mice. The other was published in Nature, investigating how mitochondrial fission factor senses and governs ferroptosis in human samples. Both citations used the antibody for Western blot (WB), spanning research fields of oncology, gut microbiology, and mitochondrial biology/ferroptosis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Cancer Res Clin Oncol; The protective effect of probiotic therapy on gut microbiota and the activation of ω-oxidation after ovarian cancer surgery.; Authors - Chen Geng
human	WB	Nature; Mitochondrial fission factor senses and governs ferroptosis; Authors - Songjun Dai