CYP4F3 Polyclonal antibody

CYP4F3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19701-1-AP

Synonyms

CPF3, CYP4F, CYP4F3, CYPIVF3, Cytochrome P450 4F3, Cytochrome P450 LTB omega, LTB4H

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Validation Data Gallery

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  WB analysis of HL-60 using 19701-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19701-1-AP (CYP4F3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19701-1-AP targets CYP4F3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 3
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000896
• Gene Symbol: CYP4F3
• Gene ID (NCBI): 4051
• RRID: AB_2878599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F3, also named as LTB4H and CYPIVF3, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F3 requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.