CYP7A1 Recombinant antibody

CYP7A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86045-2-RR
Clone No.250554G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, CP7A, CYP7, CYPVII

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, L02 cells, HuH-7 cells, SMMC-7721 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86045-2-RR targets CYP7A1 in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP7A1 fusion protein Ag12572 Predict reactive species
Full Name cytochrome P450, family 7, subfamily A, polypeptide 1
Calculated Molecular Weight 504 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC101777
Gene Symbol CYP7A1
Gene ID (NCBI) 1581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

Protocols

Product Specific Protocols
WB protocol for CYP7A1 antibody 86045-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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