CYP7B1 Polyclonal antibody, PBS Only

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• CYP7B1 Polyclonal antibody, PBS Only (24889-1-PBS)

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  WB analysis of A431 using 24889-1-AP (same clone as 24889-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis using 24889-1-AP (same clone as 24889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24889-1-AP (same clone as 24889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 24889-1-AP (same clone as 24889-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24889-1-PBS in a different storage buffer formulation.

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3, CP7B

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Product Information

24889-1-PBS targets CYP7B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19374

  Product name: Recombinant human CYP7B1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 96-403 aa of BC136574

  Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

• Full Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Calculated Molecular Weight: 506 aa, 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC136574
• Gene Symbol: CYP7B1
• Gene ID (NCBI): 9420
• RRID: AB_2879780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75881
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.