CYTSA Polyclonal antibody, PBS Only

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• CYTSA Polyclonal antibody, PBS Only (25390-1-PBS)

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  WB analysis of NIH/3T3 using 25390-1-AP (same clone as 25390-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25390-1-AP (same clone as 25390-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 25390-1-AP (same clone as 25390-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

CYTSA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 25390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

25390-1-PBS targets CYTSA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21896

  Product name: Recombinant human CYTSA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-352 aa of BC136471

  Sequence: MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEV

• Full Name: cytospin A
• Calculated Molecular Weight: 1117 aa, 125 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC136471
• Gene Symbol: CYTSA
• Gene ID (NCBI): 23384
• RRID: AB_2880056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q69YQ0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.