Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

2A2C6

Cat no : 66903-1-Ig

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

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Validation Data Gallery

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  WB analysis using 66903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66903-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin.

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  IF Staining of HepG2 using 66903-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66903-1-Ig (Calnexin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: HeLa cells, HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

66903-1-Ig targets Calnexin in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag25535
• Full Name: calnexin
• Calculated molecular weight: 90 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC003552
• Gene symbol: CANX
• Gene ID (NCBI): 821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Publications

Species	Application	Title
mouse	WB	Chin Med J (Engl); Macrophage exosomes transfer angiotensin II type 1 receptor to lung fibroblasts mediating bleomycin-induced pulmonary fibrosis.; Authors - Na-Na Sun
human	WB	Biochim Biophys Acta Mol Cell Res; IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.; Authors - Xueqiang Peng
human	WB	Front Microbiol; ANKS4B Restricts Replication of Zika Virus by Downregulating the Autophagy.; Authors - Quanshi Lin
mouse	WB	Front Oncol; Osteosarcoma Cell-Derived Small Extracellular Vesicles Enhance Osteoclastogenesis and Bone Resorption Through Transferring MicroRNA-19a-3p.; Authors - Tingting Luo
human	WB	Front Cell Dev Biol; Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter.; Authors - Yan Lu
pig		PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu