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Calnexin Monoclonal antibody
Calnexin Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66903-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells|
|Positive IHC detected in||human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66903-1-Ig targets Calnexin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Calnexin fusion protein Ag25535|
|Calculated molecular weight||90 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC003552|
|Gene ID (NCBI)||821|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Amy (Verified Customer) (11-08-2023)
Clean antibody with strong band for WB in HEK293T lysates.