Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A2C6

Cat no : 66903-1-Ig

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66903-1-Ig targets Calnexin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calnexin fusion protein Ag25535
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003552
Gene symbol CANX
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
WB protocol for Calnexin antibody 66903-1-IgDownload protocol
IHC protocol for Calnexin antibody 66903-1-IgDownload protocol
IF protocol for Calnexin antibody 66903-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
human,mouseWB,IF

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
humanWB

Cell Chem Biol

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Authors - Nan Qiu
humanIF

Diabetes

PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.

Authors - Chenrui Li
humanWB

J Nanobiotechnology

Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612

Authors - Lite Ge
pig

PLoS Pathog

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

Authors - Chun-Chun Liu