Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A2C6

Cat no : 66903-1-Ig

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90



Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

66903-1-Ig targets Calnexin in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calnexin fusion protein Ag25535
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003552
Gene symbol CANX
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
WB protocol for Calnexin antibody 66903-1-IgDownload protocol
IF protocol for Calnexin antibody 66903-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Chin Med J (Engl)

Macrophage exosomes transfer angiotensin II type 1 receptor to lung fibroblasts mediating bleomycin-induced pulmonary fibrosis.

Authors - Na-Na Sun
humanWB

Biochim Biophys Acta Mol Cell Res

IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.

Authors - Xueqiang Peng
humanWB

Front Microbiol

ANKS4B Restricts Replication of Zika Virus by Downregulating the Autophagy.

Authors - Quanshi Lin
mouseWB

Front Oncol

Osteosarcoma Cell-Derived Small Extracellular Vesicles Enhance Osteoclastogenesis and Bone Resorption Through Transferring MicroRNA-19a-3p.

Authors - Tingting Luo
humanWB

Front Cell Dev Biol

Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter.

Authors - Yan Lu
humanWB

Cell Chem Biol

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Authors - Nan Qiu