Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

CloneNo.

2A2C6

Cat no : 66903-1-Ig

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

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Validation Data Gallery

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  WB analysis using 66903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 66903-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 66903-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66903-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin.

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  IF Staining of HepG2 using 66903-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66903-1-Ig (Calnexin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 19 publications below
• IF: See 8 publications below

Product Information

66903-1-Ig targets Calnexin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag25535
• Full Name: calnexin
• Calculated molecular weight: 90 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC003552
• Gene symbol: CANX
• Gene ID (NCBI): 821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Publications

Species	Application	Title
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Cell Chem Biol; Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.; Authors - Nan Qiu
human	IF	Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li
human	WB	J Nanobiotechnology; Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612; Authors - Lite Ge
pig		PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu