Calnexin Recombinant antibody

Calnexin Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3K18

Cat no : 81938-1-RR

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81938-1-RR targets Calnexin in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Calnexin fusion protein Ag0697
Full Name calnexin
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC003552
Gene Symbol CANX
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
WB protocol for Calnexin antibody 81938-1-RRDownload protocol
IHC protocol for Calnexin antibody 81938-1-RRDownload protocol
IF protocol for Calnexin antibody 81938-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols