Calretinin antibody (12278-1-PBS)

Filter: AllWBIPIHC

WB analysis using 12278-1-AP (same clone as 12278-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of human ileum using 12278-1-AP (same clone as 12278-1-PBS)
human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 12278-1-AP (same clone as 12278-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

12278-1-PBS targets Calretinin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calretinin fusion protein Ag2924 Predict reactive species
Full Name	calbindin 2
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC015484
Gene Symbol	Calretinin
Gene ID (NCBI)	794
RRID	AB_2228338
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22676
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.