Calretinin Polyclonal antibody, PBS Only

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  WB analysis using 12278-1-AP (same clone as 12278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 12278-1-AP (same clone as 12278-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12278-1-AP (same clone as 12278-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

  IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 12278-1-AP (same clone as 12278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12278-1-PBS in a different storage buffer formulation.

Calretinin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12278-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CALB2, 29 kDa calbindin, CAB29, CAL2, calbindin 2

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Product Information

12278-1-PBS targets Calretinin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calretinin fusion protein Ag2924
• Full Name: calbindin 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015484
• Gene Symbol: Calretinin
• Gene ID (NCBI): 794
• RRID: AB_2228338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22676
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.