Tested Applications
| Positive WB detected in | SH-SY5Y cells, U2OS cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue |
| Positive IHC-Autostainer detected in | human brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5100 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82811-1-RR targets Calretinin in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2924 Product name: Recombinant human Calretinin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC015484 Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM Predict reactive species |
| Full Name | calbindin 2 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC015484 |
| Gene Symbol | Calretinin |
| Gene ID (NCBI) | 794 |
| RRID | AB_3670560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22676 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Calretinin antibody 82811-1-RR | Download protocol |
| IF protocol for Calretinin antibody 82811-1-RR | Download protocol |
| IHC protocol for Calretinin antibody 82811-1-RR | Download protocol |
| WB protocol for Calretinin antibody 82811-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calretinin Recombinant monoclonal antibody (2F20), catalog number 82811-1-RR, has 4 citations. These appear in Cell Death Dis, Clinical Epigenetics, Cell and Tissue Research, and Medical International. The research fields span gastric cancer peritoneal metastasis, uterine adenomatoid tumor genomics, cochlear nuclei development, and ovarian fibroma pathology. Applications cited include immunohistochemistry and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Tumor exosomal circPTBP3 drives gastric cancer peritoneal metastasis via mesothelial-mesenchymal transition | ||
Clin Epigenetics Genomic characterization of uterine adenomatoid tumors reveals recurrent alterations in KMT5A and KMT2C. | ||
Cell Tissue Res An Lmx1a/b allelic series reveals the role of Lmx1 genes in cochlear nuclei development. | ||
Med Int (Lond) Ovarian fibroma torsion mimicking solid malignant tumors of the ovary: A case report and mini-review of the literature. |
