Calumenin Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 67585-1-Ig (same clone as 67585-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67585-1-Ig (CALU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67585-1-Ig (same clone as 67585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (67585-1-Ig, Clone: 2D8B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67585-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50698-2

  Cytometric bead array standard curve of MP50698-2, CALU Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67585-2-PBS. Detection antibody: 67585-1-PBS. Standard:Ag12073. Range: 0.195-100 ng/mL.

Calumenin Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67585-1-PBS

Clone No.2D8B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CALU, 2D8B8, Crocalbin, IEF SSP 9302

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Product Information

67585-1-PBS targets Calumenin as part of a matched antibody pair:

MP50698-2: 67585-2-PBS capture and 67585-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12073

  Product name: Recombinant human CALU protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC013383

  Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF

• Full Name: calumenin
• Calculated Molecular Weight: 315 aa, 37 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC013383
• Gene Symbol: Calumenin
• Gene ID (NCBI): 813
• RRID: AB_2882794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43852
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.