Calumenin Monoclonal antibody

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  WB analysis using 67585-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67585-1-Ig (CALU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 67585-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67585-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (67585-1-Ig, Clone: 2D8B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Calumenin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67585-1-Ig

Clone No.2D8B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CALU, 2D8B8, Crocalbin, IEF SSP 9302

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, Jurkat cells, RAW264.7 cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue, human kidney tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67585-1-Ig targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12073

  Product name: Recombinant human CALU protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC013383

  Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF

• Full Name: calumenin
• Calculated Molecular Weight: 315 aa, 37 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC013383
• Gene Symbol: Calumenin
• Gene ID (NCBI): 813
• RRID: AB_2882794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43852
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.