Recombinant Canine IL-6 protein (rFc Tag)

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• Recombinant Canine IL-6 protein (rFc Tag) (Eg4554)

Species

Canine

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4554

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Validation Data Gallery

• 

  Purity of Recombinant Canine IL-6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Canine IL-6 protein Phe21-Met207 (Accession# P41323) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 403985
• Accession: P41323
• PredictedSize: 48.1 kDa
• SDS-PAGE: 48-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro- and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types, including T cells and macrophages, as a phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the terminal differentiation of B cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces the growth of myeloma and plasmacytoma and the differentiation of neurons and acts on B cells, T cells, hepatocytes, haematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced by muscle that is increased in response to muscle contraction. IL-6 has been shown to interact with the interleukin-6 receptor and glycoprotein 130. In addition, IL-6 has been implicated in haematopoiesis, bone metabolism and cancer progression, and has been defined as essential in directing the transition from innate to acquired immunity.

References:

1.Aliyu M. et al. (2022) Int Immunopharmacol. 111:109130.
2.Grebenciucova E. et al. (2023) Front Immunol. 14:1255533.
3.Tanaka T. et al. (2016) Adv Exp Med Biol. 941:79-88.