Recombinant Canine SCF protein (rFc Tag)

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• Recombinant Canine SCF protein (rFc Tag) (Eg4548)

Species

Canine

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4548

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Validation Data Gallery

• 

  Purity of Recombinant Canine SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Canine SCF protein Lys26-Gln215 (Accession# Q06220) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 403507
• Accession: Q06220
• PredictedSize: 47.3 kDa
• SDS-PAGE: 50-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Stem cell factor (SCF), also known as mast cell growth factor, steel factor and Kit ligand, is a haematopoietic growth factor. SCF is a critical protein with key roles in the cell such as haematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signalling pathways by binding to the c-kit receptor. SCF and its receptor, c-kit ligand (KL), are particularly upregulated in human malignancies, including gastrointestinal stromal tumours (GISTs), breast cancer, haematopoietic malignancies, myeloid leukaemia and gliomas. The SCF/c-KIT system also plays an important role in cell fate decision and its activity is known to control cell proliferation, differentiation, survival and apoptosis.

References:

1.Vazquez-Mellado MJ. et al. (2017) Vitam Horm. 105:273-296.
2.Figueira MI. et al. (2017) Cancer Lett. 405:10-21.
3.Yasuda A. et al. (2006) Mol Cancer. 5:46.