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Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody, PBS Only (84233-8-PBS)

Filter: AllIHCAffinity

IHC staining of human kidney(renal cell) cancer using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
IHC staining of human kidney(renal cell) cancer using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
IHC staining of human kidney using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
IHC staining of human kidney using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 84233-8-RR (same clone as 84233-8-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-8-PBS in a different storage buffer formulation.
Affinity of 84233-8-RR
Biolayer interferometry (BLl) kinetic assays of 84233-8-RR against Human Carbonic Anhydrase IX/CA9 were performed. The affinity constant is 0.469 nM.

Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody, PBS Only

Name: Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 84233-8-PBS

Carbonic Anhydrase IX/CA9 Uni-rAb^® Recombinant Antibody for IHC, Indirect ELISA

Cat No. 84233-8-PBS

Clone No.241573F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84233-8-PBS targets Carbonic Anhydrase IX/CA9 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag36941 Product name: Recombinant human CA9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-408 aa of BC014950 Sequence: LMPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS Predict reactive species
Full Name	carbonic anhydrase IX
Calculated Molecular Weight	459 aa, 50 kDa
GenBank Accession Number	BC014950
Gene Symbol	CA9
Gene ID (NCBI)	768
RRID	AB_3743534
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16790
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)