Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody (84233-8-RR)

• 

  IHC staining of human kidney(renal cell) cancer using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney(renal cell) cancer using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) slide using 84233-8-RR (CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 84233-8-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84233-8-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 84233-8-RR

  Biolayer interferometry (BLl) kinetic assays of 84233-8-RR against Human Carbonic Anhydrase IX/CA9 were performed. The affinity constant is 0.469 nM.

Carbonic Anhydrase IX/CA9 Uni-rAb^® Recombinant Antibody for IHC, ELISA

Cat No. 84233-8-RR

Clone No.241573F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

---

Tested Applications

• Positive IHC detected in: human kidney(renal cell) cancer, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84233-8-RR targets Carbonic Anhydrase IX/CA9 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag36941

  Product name: Recombinant human CA9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 32-408 aa of BC014950

  Sequence: LMPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS

• Full Name: carbonic anhydrase IX
• Calculated Molecular Weight: 459 aa, 50 kDa
• GenBank Accession Number: BC014950
• Gene Symbol: CA9
• Gene ID (NCBI): 768
• RRID: AB_3743534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)