Carboxypeptidase D/CPD Recombinant monoclonal antibody

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• Carboxypeptidase D/CPD Recombinant monoclonal antibody (83229-5-RR)

• 

  WB analysis using 83229-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83229-5-RR (CBPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83229-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83229-5-RR (CBPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Carboxypeptidase D/CPD Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83229-5-RR

Clone No.260182E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CPD, carboxypeptidase D, GP180

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Tested Applications

• Positive WB detected in: TT cells, HEK-293 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83229-5-RR targets Carboxypeptidase D/CPD in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5677

  Product name: Recombinant human CBPD protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 32-1299 aa of NM_001304.4

  Sequence: AHIKKAEATTTTTSAGAEAAEGQFDRYYHEEELESALREAAAAGLPGLARLFSIGRSVEGRPLWVLRLTAGLGSLIPEGDAGPDAAGPDAAGPLLPGRPQVKLVGNMHGDETVSRQVLIYLARELAAGYRRGDPRLVRLLNTTDVYLLPSLNPDGFERAREGDCGFGDGGPSGASGRDNSRGRDLNRSFPDQFSTGEPPALDEVPEVRALIEWIRRNKFVLSGNLHGGSVVASYPFDDSPEHKATGIYSKTSDDEVFKYLAKAYASNHPIMKTGEPHCPGDEDETFKDGITNGAHWYDVEGGMQDYNYVWANCFEITLELSCCKYPPASQLRQEWENNRESLITLIEKVHIGVKGFVKDSITGSGLENATISVAGINHNITTGRFGDFYRLLVPGTYNLTVVLTGYMPLTVTNVVVKEGPATEVDFSLRPTVTSVIPDTTEAVSTASTVAIPNILSGTSSSYQPIQPKDFHHHHFPDMEIFLRRFANEYPNITRLYSLGKSVESRELYVMEISDNPGVHEPGEPEFKYIGNMHGNEVVGRELLLNLIEYLCKNFGTDPEVTDLVHNTRIHLMPSMNPDGYEKSQEGDSISVIGRNNSNNFDLNRNFPDQFVQITDPTQPETIAVMSWMKSYPFVLSANLHGGSLVVNYPFDDDEQGLATYSKSPDDAVFQQIALSYSKENSQMFQGRPCKNMYPNEYFPHGITNGASWYNVPGGMQDWNYLQTNCFEVTIELGCVKYPLEKELPNFWEQNRRSLIQFMKQVHQGVRGFVLDATDGRGILNATISVAEINHPVTTYKTGDYWRLLVPGTYKITASARGYNPVTKNVTVKSEGAIQVNFTLVRSSTDSNNESKKGKGASSSTNDASDPTTKEFETLIKDLSAENGLESLMLRSSSNLALALYRYHSYKDLSEFLRGLVMNYPHITNLTNLGQSTEYRHIWSLEISNKPNVSEPEEPKIRFVAGIHGNAPVGTELLLALAEFLCLNYKKNPAVTQLVDRTRIVIVPSLNPDGRERAQEKDCTSKIGQTNARGKDLDTDFTNNASQPETKAIIENLIQKQDFSLSVALDGGSMLVTYPYDKPVQTVENKETLKHLASLYANNHPSMHMGQPSCPNKSDENIPGGVMRGAEWHSHLGSMKDYSVTYGHCPEITVYTSCCYFPSAARLPSLWADNKRSLLSMLVEVHKGVHGFVKDKTGKPISKAVIVLNEGIKVQTKEGGYFHVLLAPGVHNIIAIADGYQQQHSQVFVHHDAASSVVIVFDTDNRIFGLPRE

• Full Name: carboxypeptidase D
• Calculated Molecular Weight: 153 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: NM_001304.4
• Gene Symbol: CPD
• Gene ID (NCBI): 1362
• RRID: AB_3743471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75976-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carboxypeptidase D (CPD) is a membrane-bound metallocarboxypeptidase that processes proteins and peptides during the secretory pathway, particularly by removing C-terminal arginine or lysine residues. Encoded by the CPD gene, it plays a regulatory role in peptide hormone processing and is also known to be a receptor for hepatitis B virus. Functioning as a regulatory type B carboxypeptidase, it typically acts on substrates within the trans-Golgi network and is recycled to the plasma membrane. With a predicted molecular weight of 152 kDa, CPD, as a membrane-bound glycoprotein, undergoes significant glycosylation modifications. The actual observed molecular weight is usually higher than the theoretical value; 180-200 kDa represents the typical apparent molecular weight range. This difference is primarily due to N-linked and O-linked glycosylations.