Carboxypeptidase M Recombinant monoclonal antibody

• 

  WB analysis using 87661-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87661-1-RR (CBPM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87661-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87661-1-RR (CBPM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87661-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87661-1-RR against Human Carboxypeptidase M were performed. The affinity constant is 0.223 nM.

Carboxypeptidase M Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87661-1-RR

Clone No.252972G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CPM, CarboxypeptidaseM, EC:3.4.17.12

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human adipose tissue, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87661-1-RR targets Carboxypeptidase M in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5655

  Product name: Recombinant human CBPM protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 18-423 aa of NM_198320.5

  Sequence: LDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHS

• Full Name: carboxypeptidase M
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa, 62-70 kDa
• GenBank Accession Number: NM_198320.5
• Gene Symbol: Carboxypeptidase M
• Gene ID (NCBI): 1368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14384
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.