Cardiac Troponin I Recombinant monoclonal antibody, PBS Only

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• Cardiac Troponin I Recombinant monoclonal antibody, PBS Only (86791-1-PBS)

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  WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Negative control: mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue. This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

  Mouse heart tissue were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 86791-1-RR (same clone as 86791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86791-1-RR (same clone as 86791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cardiac Troponin I antibody (86791-1-RR, Clone: 251696H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

Cardiac Troponin I Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86791-1-PBS

Clone No.251696H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

cTnI, TNNI3

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Product Information

86791-1-PBS targets Cardiac Troponin I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16365

  Product name: Recombinant human TNNI3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-110 aa of BC096165

  Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE

• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• RRID: AB_3745120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19429
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes.