Cardiac Troponin I Recombinant monoclonal antibody

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• Cardiac Troponin I Recombinant monoclonal antibody (86791-1-RR)

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  WB analysis using 86791-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Negative control: mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue.

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  WB analysis using 86791-1-RR

  Mouse heart tissue were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 86791-1-RR

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 86791-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cardiac Troponin I antibody (86791-1-RR, Clone: 251696H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cardiac Troponin I Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 86791-1-RR

Clone No.251696H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

cTnI, TNNI3

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Tested Applications

• Positive WB detected in: human heart tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86791-1-RR targets Cardiac Troponin I in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16365

  Product name: Recombinant human TNNI3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-110 aa of BC096165

  Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE

• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• RRID: AB_3745120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19429
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes.