Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26592-1-AP

Synonyms

Cardiac muscle troponin T, Cardiac Troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26592-1-AP targets Cardiac Troponin T in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag24911
Full Name troponin T type 2 (cardiac)
Calculated molecular weight 36 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC002653
Gene symbol TNNT2
Gene ID (NCBI) 7139
RRIDAB_2880566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
IHC protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
IF protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomaterials

Targeted immunomodulation therapy for cardiac repair by platelet membrane engineering extracellular vesicles via hitching peripheral monocytes.

Authors - Qiyu Li
humanIF

Free Radic Biol Med

SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics

Authors - Rui Wang
mouseIHC

Front Physiol

The physiological response during optogenetic-based cardiac pacing in awake freely moving mice

Authors - Jun Kaminosono