Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26592-1-AP

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26592-1-AP is Cardiac Troponin T Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag24911
Full Name troponin T type 2 (cardiac)
Calculated molecular weight 36 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC002653
Gene symbol TNNT2
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
IHC protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
IF protocol for Cardiac Troponin T antibody 26592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols