Casein Kinase 1 Delta Recombinant monoclonal antibody

• 

  WB analysis using 86423-2-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86423-2-RR (CSNK1D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Casein Kinase 1 Delta Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86423-2-RR

Clone No.251238D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

casein kinase 1, delta, CKId, CKI-delta, CSNK1D, EC:2.7.11.1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, K-562 cell, PANC-1 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86423-2-RR targets Casein Kinase 1 Delta in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5856

  Product name: Recombinant human CSNK1D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 103-373 aa of BC003558

  Sequence: KTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRL

• Full Name: casein kinase 1, delta
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC003558
• Gene Symbol: CK1 Delta
• Gene ID (NCBI): 1453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48730
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.