Caspase 1 Recombinant monoclonal antibody

• 

  WB analysis using 84735-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84735-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 84735-1-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Caspase 1 Recombinant antibody (84735-1-RR, Clone:241967A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Caspase 1 Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84735-1-RR

Clone No.241967A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

---

Tested Applications

• Positive WB detected in: THP-1 cells, U-937 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84735-1-RR targets Caspase 1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC062327
• Gene Symbol: Caspase 1
• Gene ID (NCBI): 834
• RRID: AB_3672119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P29466
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.