Caspase 1/P20 Polyclonal antibody, PBS Only

Name: Caspase 1/P20 Polyclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 22915-1-PBS-100UG

Caspase 1/P20 Polyclonal Antibody for WB, IHC, IF, IF/ICC, ELISA

Cat No. 22915-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IF/ICC, ELISA

Synonyms

CASP1, Caspase1, CASP 1, CASP-1, Caspase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBIHCIF/ICC

WB analysis using 22915-1-AP (same clone as 22915-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 22915-1-AP (same clone as 22915-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 22915-1-AP (same clone as 22915-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 22915-1-AP (same clone as 22915-1-PBS)
WB result of Caspase 1/P20 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HUVEC using 22915-1-AP (same clone as 22915-1-PBS)
IF result of 22915-1-AP from publication with PMID: 31611559. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

22915-1-PBS targets Caspase 1/P20 in WB, IHC, IF, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11143 Product name: Recombinant human Caspase 1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-311 aa of BC062327 Sequence: MADKVLKEKRKLFIRSMGEAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH Predict reactive species
Full Name	caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Calculated Molecular Weight	404 aa, 45 kDa
Observed Molecular Weight	45-47 kDa, 30 kDa, 35 kDa
GenBank Accession Number	BC062327
Gene Symbol	Caspase 1
Gene ID (NCBI)	834
RRID	AB_2876874
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29466
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.